CTLA4 Polyclonal Antibody

SKU: AN006100L

Size::25μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light.

Shipping:The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Polyclonal Antibodies

Abbreviation:CTLA4

Target Synonym:Ctla4;Ly-56;GRD;Cytotoxic T-lymphocyte protein;Cytotoxic T-lymphocyte-associated antigen;IDDM;CELIAC;CELIAC3;CTLA-4;Cytotoxic T-lymphocyte protein 4;Cytotoxic T-lymphocyte-associated antigen 4;GRD4;GSE;IDDM12;Cd152;Ctla4; Ly-56

Research Areas:

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human

Application:IHC

Isotype:IgG

Clonality:Polyclonal

Clone NO.:

UNIProt ID:P09793

Accession:

Background:CTLA-4 and CD28, together with their ligands B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. CTLA-4 and CD28 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. Both CTLA-4 and CD28 are composed of a single Ig V‑like extracellular domain, a transmembrane domain and an intracellular domain. CTLA-4 and CD28 are both expressed on the cell surface as disulfide-linked homodimers or as monomers. The genes encoding these two molecules are closely linked on human chromosome 2. CTLA-4 was originally identified as a gene that was specifically expressed by cytotoxic T lymphocytes. However, CTLA-4 transcripts have since been found in both Th1 and Th2, and CD4+ and CD8+ T cell clones. Whereas, CD28 expression is constitutive on the surfaces of 95% of CD4+ T cells and 50% of CD8+ T cells and is down regulated upon T cell activation, CTLA-4 expression is upregulated rapidly following T cell activation and peaks approximately 24 hours following activation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with 20-100-fold higher affinity than CD28. The physiological role of CTLA-4 in T cell costimulation is currently being studied.

Concentration:1 mg/mL

Immunogen:Recombinant Mouse CTLA4 protein expressed by Mammalian

Buffer:PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4

Purification method:Antigen Affinity Purification

Dilution:IHC 1:500-1:1000

Calculated MW:

ObservedMW: