The picture shows the comparison between ssDNA 100 Ladder and single-stranded DNA with lengths of 150, 280, and 605 bases on 8% PAGE denaturing gel.
Item number and specifications: B1003-100uL
Description
ssDNA 100 Ladder is a single-stranded DNA ruler with an increment of 100 bases. The shortest band corresponds to 100 nt, and the longest band exceeds 1500 nt. Samples were stored in 100 uL buffer containing: 50 mM HEPES (pH ~7), 100 mM NaCl, 2 mM ZnCl2, 2 mM EDTA. The total DNA concentration is approximately 20 ng/uL. ssDNA 100 Ladder is suitable for running denaturing PAGE gels, and SYBR Gold Nucleic Acid Gel Stain has the best staining effect.
Recommended protocol: Mix 3-5 uL ssDNA 100 Ladder sample with denatured Loading Buffer and load the sample. After running the gel, soak and stain with SYBR Gold Nucleic Acid Gel Stain for 10-20 minutes. The 5' end of ssDNA 100 Ladder contains -PO43-, and the 5' end can be labeled with fluorescence or 32P after removing this group.
Instructions and Precautions: SYBR Gold Nucleic Acid Gel Stain has the best binding effect on single-stranded nucleic acids. It is strongly recommended to use SYBR Gold staining to obtain the best gel pattern.
Reference: Gu, H. and Breaker, R. R. Production of single-stranded DNAs by self-cleavage of rolling-circle amplification products. BioTechniques 54: 337-343 (June 2013) doi 10.2144/000114009