Canine Cytochrome P450 19A1,CYP19A1 ELISA KIT

SKU: E0063Ca

Size:96 Tests-1 Kit

Background:A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). {ECO:0000250|UniProtKB:P11511}.

Research_area:

Applications:ELISA

Target_protein:CYP19A1

Reactivity:Canine

Assay type:Sandwich

Sensitivity:7.52ng/L

Detection range:15-3000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Cytochrome P450 19A1

Species:Canine

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q5QQX7

MASS(Da):58084

GeneID:494003

Synonyms:CYP19A1 CYP19

Gene names:CYP19A1

Standard solution:3200ng/L