C1q ELISA kit (Human)

SKU: SEA747Hu

Size: 96Tests

# of Times Cited in literature: 7

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: C1q

Target Full Name: Complement 1q

Alternative Names: C1-q; Autoantigen Clq

Target Species: Human

Uniprot: P02745 & P02746 & P02747

Gene ID: 712 & 713 & 714

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Human C1q / Complement 1q

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 0.156-10ng/mL

Sensitivity: 0.056ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement 1q (C1q) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement 1q (C1q) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Complement 1q (C1q). No significant cross-reactivity or interference between Complement 1q (C1q) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement 1q (C1q). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement 1q (C1q). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement 1q (C1q), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement 1q (C1q) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Infection immunity;Immune molecule;Autoimmunity;

References Citing This Product: Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease.

Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis

The role of complement components C1q, MBL and C1 inhibitor in pathogenesis of endometriosis

Intrathecal complement activation by the classical pathway in tick-borne encephalitis

Complement activation profile of patients with primary focal segmental glomerulosclerosis

Plasma and urine complement activation products in light chain and heavy chain deposition disease

Elevated Serum Complement C1q Levels After Traumatic Brain Injury and Its Association with Poor Prognosis