Bovine Ubiquitin-like Protein Isg15, ISG15 ELISA Kit

SKU: E0359Bo

Size:96 Tests-1 Kit

Background:Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Exhibits antiviral activity towards both DNA and RNA viruses. The secreted form of ISG15 can: induce natural killer cell proliferation, augment lymphokine-activated-killer (LAK) activity, induce dendritic cell maturation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity (By similarity). The secreted form acts through the integrin ITGAL/ITGB2 receptor to initiate activation of SRC family tyrosine kinases including LYN, HCK and FGR which leads to secretion of IFNG and IL10; the interaction is mediated by ITGAL (By similarity). In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy (PubMed:9546718).

Research_area:Others

Applications:ELISA

Target_protein:ISG15

Reactivity:Bovine

Assay type:Sandwich

Sensitivity:2.45ng/L

Detection range:5-2000ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Ubiquitin-like protein ISG15

Species:Bovine

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:O02741

MASS(Da):17,310

GeneID:281871

Synonyms:BoUCRP;Interferon-stimulated gene product 17;ISG15;Ubiquitin cross-reactive protein;Ubiquitin-like protein ISG15

Gene names:ISG15

Standard solution:2400ng/L