Bovine Nuclear factor erythroid 2-related factor 2,NRF2 ELISA KIT

SKU: E0189Bo

Size:96 Tests-1 Kit

Background:Transcription factor that plays a key role in the response to oxidative stress: binds to antioxidant response (ARE) elements present in the promoter region of many cytoprotective genes, such as phase 2 detoxifying enzymes, and promotes their expression, thereby neutralizing reactive electrophiles. In normal conditions, ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex. In response to oxidative stress, electrophile metabolites inhibit activity of the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2, heterodimerization with one of the small Maf proteins and binding to ARE elements of cytoprotective target genes. The NFE2L2/NRF2 pathway is also activated in response to selective autophagy: autophagy promotes interaction between KEAP1 and SQSTM1/p62 and subsequent inactivation of the BCR(KEAP1) complex, leading to NFE2L2/NRF2 nuclear accumulation and expression of cytoprotective genes (By similarity). May also be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region (By similarity). {ECO:0000250|UniProtKB:Q16236, ECO:0000250|UniProtKB:Q60795}.

Research_area:

Applications:ELISA

Target_protein:NFE2L2

Reactivity:Bovine

Assay type:Sandwich

Sensitivity:5.19ng/L

Detection range:10-640ng/L

Sample type:Serum, plasma or other biological fluids

Full product name:Nuclear factor erythroid 2-related factor 2

Species:Bovine

Storage:2-8ºC

Assay time:1h 30m

UniProt accession:Q5NUA6

MASS(Da):67895

GeneID:

Synonyms:NFE2L2 NRF2

Gene names:NFE2L2

Standard solution:1280ng/L