BLM (phospho Thr99) rabbit pAb

SKU: ES7149

Size: 100μL

Source:Rabbit

Applications:WB;IHC;IF;ELISA

Reactivity:Human;Rat;Mouse;

Dilution:Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Immunogen:The antiserum was produced against synthesized peptide derived from human Bloom Syndrome around the phosphorylation site of Thr99. AA range:65-114

Storage_stability:-20°C/1 year

Clonality:Polyclonal

Isotype:IgG

Concentration:1 mg/ml

Observed_band(KD):159kD

Human_gene_id:641

Human_swiss_prot_no:P54132

Subcellular_location:Nucleus . Together with SPIDR, is redistributed in discrete nuclear DNA damage-induced foci following hydroxyurea (HU) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a RMI complex- and SPIDR-dependent manner.

Other_name:BLM; RECQ2; RECQL3; Bloom syndrome protein; DNA helicase; RecQ-like type 2; RecQ2; RecQ protein-like 3

Background:The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq, Jul 2008],