BL21 (DE3) pLysS Chemically Competent Cell

SKU: CD701

Features

• Transformation efficiency: >107 cfu/μg (pUC19 DNA).

• CamR.

• Contains pLysS plasmid that expresses the T7 lysozyme gene to reduce the background of the target protein expression without

disturbing IPTG functioning.

• Suitable for non-toxic and toxic protein’s expression.

• Control plasmid I (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.

Storage

At -70°C for six months. Do not store in liquid nitrogen.

Papers cited the use of this product:

Literature	Journal	IF	Author	Date	Link
A single fluorescent protein-based sensor for in vivo 2-oxogluatarate detection in cell	Biosensors and Bioelectronics	5.437	Chang Zhang, et al. Lab of Biosystems and Microana	2014 Apr	The original link
A Dimerization-Dependent Mechanism Drives the Endoribonuclease Function of PRRSV nsp11	J Virol.	4.439	Yuejun Shi, et al.	2016 Feb	The original link
Quantitative monitoring of 2-oxoglutarate in Escherichia coli cells by a fluorescence resonance energy transfer-based biosensor	Appl Microbiol Biotechnol	3.689	Chang Zhang, et al. Laboratory of Biosystems and M	2013 Jul	The original link
Amino acid residue Y196E substitution and C-terminal peptide synergistically alleviate the toxicity of Clostridium perfringens epsilon toxin	Toxicon	2.492	Wenwu Yao, et al.	2015 Apr	The original link