Anti-XIAP-associated factor 1 XAF1 Antibody Picoband®

SKU: PA1218

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: XAF1

Specificity: No cross reactivity with other proteins.

Background: XIAP associated factor-1, also known as XAF1, is a human gene. X-linked inhibitor of apoptosis (XIAP; MIM 300079) is a potent member of the IAP family. All members of this family possess baculoviral IAP (BIR) repeats, cysteine-rich domains of approximately 80 amino acids that bind and inhibit caspases. XAF1 antagonizes the anticaspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells. And alteration in XAF1 and XIAP RNA expression levels may lead to increased apoptotic resistance and proliferation due to unregulated XIAP function in cancer cells.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human XAF1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Fong, W. G.; Liston, P.; Rajcan-Separovic, E.; St. Jean, M.; Craig, C.; Korneluk, R. G. : Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines. Genomics 70: 113-122, 2000. 2. Liston, P.; Fong, W. G.; Kelly, N. L.; Toji, S.; Miyazaki, T.; Conte, D.; Tamai, K.; Craig, C. G.; McBurney, M. W.; Korneluk, R. G. : Identification of XAF1 as an antagonist of XIAP anti-caspase activity. Nature Cell Biol. 3: 128-133, 2001.

Uniprot ID: Q6GPH4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 35 kDa

Calculated Molecular Weight: 34626 MW

Gene ID: 54739

Protein Name: XIAP-associated factor 1

Gene Full Name: XIAP associated factor 1

Synonyms: XAF1; BIRC4BP; XIAPAF1; BIRC4-binding protein