Anti-Vaspin/SERPINA12 Antibody Picoband®

SKU: A05352-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SERPINA12

Specificity:

Background: Serpin A12 (serine proteinase inhibitor-clade A #12; also vaspin) is a 45?50 kDa, secreted member of the alpha1-antitrypsin (clade A)-subfamily, Serpin superfamily of protease inhibitors. It is produced by visceral and subcutaneous adipocytes, plus adipocyte-derived stem cells during obesity or diabetes. The presence of vaspin reverses insulin-resistance. Its molecular target is unknown, but it may antagonize the action of proteases that impair the action of insulin. Rat Serpin A12 is 411 amino acids (aa) in length. It contains a 20 aa signal sequence and a 391 aa mature region. A potential “active” inhibitor site lies between Gly364 and Pro389. Mature rat Serpin A12 shares 62% and 88% aa identity with human and mouse Serpin A12, respectively.

Immunogen: E.coli-derived human SERPINA12 recombinant protein (Position: K31-L376). Human Vaspin/SERPINA12 shares 64.5% and 65.4% amino acid (aa) sequence identity with mouse and rat SERPINA12, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Heiker, J. T., Kloting, N., Kovacs, P., Kuettner, E. B., Strater, N., Schultz, S., Kern, M., Stumvoll, M., Bluher, M., Beck-Sickinger, A. G. Vaspin inhibits kallikrein 7 by serpin mechanism. Cell. Molec. Life Sci. 70: 2569-2583, 2013. 2. Hida, K., Wada, J., Eguchi, J., Zhang, H., Baba, M., Seida, A., Hashimoto, I., Okada, T., Yasuhara, A., Nakatsuka, A., Shikata, K., Hourai, S., Futami, J., Watanabe, E., Matsuki, Y., Hiramatsu, R., Akagi, S., Makino, H., Kanwar, Y. S. Visceral adipose tissue-derived serine protease inhibitor: a unique insulin-sensitizing adipocytokine in obesity. Proc. Nat. Acad. Sci. 102: 10610-10615, 2005. 3. Kloting, N., Berndt, J., Kralisch, S., Kovacs, P., Fasshauer, M., Schon, M. R., Stumvoll, M., Bluher, M. Vaspin gene expression in human adipose tissue: association with obesity and type 2 diabetes. Biochem. Biophys. Res. Commun. 339: 430-436, 2006.

Uniprot ID: Q8IW75

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 47 kDa

Calculated Molecular Weight:

Gene ID: 145264

Protein Name: Serpin A12

Gene Full Name: serpin family A member 12

Synonyms: OL 64; Serpin A12; SERPINA12; Vaspin