Anti-USP25 Antibody Picoband®

SKU: A06182-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat,Monkey

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: USP25

Specificity: No cross reactivity with other proteins.

Background: Ubiquitin specific peptidase 25 is a protein that in humans is encoded by the USP25 gene. It is mapped to 21q21.1. Ubiquitin is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25.

Immunogen: E.coli-derived human USP25 recombinant protein (Position: M1-D1053).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Meulmeester, E., Kunze, M., Hsaio, H. H., Urlaub, H., Melchior, F. Mechanism and consequences for paralog-specific sumoylation of ubiquitin-specific protease 25. Molec. Cell 30: 610-619, 2008. 2. Valero, R., Bayes, M., Sanchez-Font, M. F., Gonzalez-Angulo, O., Gonzalez-Duarte, R., Marfany, G. Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25. Genome Biol. 2: research0043, 2001. Note: Electronic Article. 3. Valero, R., Marfany, G., Gonzalez-Angulo, O., Gonzalez-Gonzalez, G., Puelles, L., Gonzales-Duarte, R. USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2. Genomics 62: 395-405, 1999.

Uniprot ID: Q9UHP3

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 125 kDa

Calculated Molecular Weight: 49655 MW

Gene ID: 29761

Protein Name: Ubiquitin carboxyl-terminal hydrolase 25

Gene Full Name: ubiquitin specific peptidase 25

Synonyms: Deubiquitinating enzyme 25; Ubiquitin thiolesterase 25; USP on chromosome 21; USP21; USP25