Anti-UCHL5IP/HAUS7 Antibody Picoband®

SKU: A12338-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: HAUS7

Specificity:

Background: This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human UCHL5IP/HAUS7 recombinant protein (Position: D18-Q333). Human HAUS7 shares 69% amino acid (aa) sequence identity with mouse HAUS7.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Goshima, G., Mayer, M., Zhang, N., Stuurman, N., Vale, R. D. Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle. J. Cell Biol. 181: 421-429, 2008. 2. Lawo, S., Bashkurov, M., Mullin, M., Gomez Ferreria, M., Kittler, R., Habermann, B., Tagliaferro, A., Poser, I., Hutchins, J. R. A., Hegemann, B., Pinchev, D., Buchholz, F., Peters, J.-M., Hyman, A. A., Gingras, A.-C., Pelletier, L. HAUS, the 8-subunit human augmin complex, regulates centrosome and spindle integrity. Curr. Biol. 19: 816-826, 2009. 3. Li, L., Sha, Y.-W., Su, Z.-Y., Mei, L.-B., Ji, Z.-Y., Zhang, Q., Lin, S.-B., Wang, X., Qiu, P.-P., Li, P., Yin, C. A novel mutation in HAUS7 results in severe oligozoospermia in two brothers. Gene 639: 106-110, 2018.

Uniprot ID: Q99871

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 46 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 55559

Protein Name: HAUS augmin-like complex subunit 7

Gene Full Name: HAUS augmin like complex subunit 7

Synonyms: HAUS7; HSXQ28ORF; UCHL5 interacting protein; UCHL5IP; UIP1; X linked protein STS1769