Anti-TSEN54 Antibody Picoband®

SKU: A07022-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: TSEN54

Specificity:

Background: This gene encodes a subunit of the tRNA splicing endonuclease complex, which catalyzes the removal of introns from precursor tRNAs. The complex is also implicated in pre-mRNA 3-prime end processing. Mutations in this gene result in pontocerebellar hypoplasia type 2.

Immunogen: E.coli-derived human TSEN54 recombinant protein (Position: D147-H507).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Barth, P. G., Aronica, E., de Vries, L., Nikkels, P. G. J., Scheper, W., Hoozemans, J. J., Poll-The, B.-T., Troost, D. Pontocerebellar hypoplasia type 2: a neuropathological update. Acta Neuropath. 114: 373-386, 2007. 2. Budde, B. S., Namavar, Y., Barth, P. G., Poll-The, B. T., Nurnberg, G., Becker, C., van Ruissen, F., Weterman, M. A. J., Fluiter, K., te Beek, E. T., Aronica, E., van der Knaap, M. S., and 26 others. tRNA splicing endonuclease mutations cause pontocerebellar hypoplasia. Nature Genet. 40: 1113-1118, 2008. 3. Cassandrini, D., Biancheri, R., Tessa, A., Di Rocco, M., Di Capua, M., Bruno, C., Denora, P. S., Sartori, S., Rossi, A., Nozza, P., Emma, F., Mezzano, P., Politi, M. R., Laverda, A. M., Zara, F., Pavone, L., Simonati, A., Leuzzi, V., Santorelli, F. M., Bertini, E. Pontocerebellar hypoplasia: clinical, pathologic, and genetic studies. Neurology 75: 1459-1464, 2010.

Uniprot ID: Q7Z6J9

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 59 kDa

Calculated Molecular Weight:

Gene ID: 283989

Protein Name: tRNA-splicing endonuclease subunit Sen54

Gene Full Name: tRNA splicing endonuclease subunit 54

Synonyms: TSEN54; SEN54; tRNA-splicing endonuclease subunit Sen54; SEN54 homolog; HsSEN54; tRNA-intron endonuclease Sen54