Anti-TRPV5 Antibody Picoband®

SKU: A03218-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IHC,IHC-F,ICC,WB

Application Details: Western blot, 0.25-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells
ELISA, 0.1-0.5μg/ml

Gene Name: TRPV5

Specificity: No cross reactivity with other proteins.

Background: Transient receptor potential cation channel subfamily V member 5is aproteinthat in humans is encoded by the TRPV5 gene. This gene is a member of the transient receptor family and the TrpV subfamily. The calcium-selective channel encoded by this gene has 6 transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. And this protein forms homotetramers or heterotetramers and is activated by a low internal calcium level. In addition, TRPV5 is mainly expressed inkidneyepithelial cells, where it plays an important role in the reabsorption of Ca2+. Genetic deletion of TRPV5 in mice leads to Ca2+loss in the urine, and consequentialhyperparathyroidism, and bone loss.

Immunogen: E.coli-derived human TRPV5 recombinant protein (Position: Q14-Q74+H636-F729).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Clapham DE, Julius D, Montell C, Schultz G (Dec 2005). International Union of Pharmacology. XLIX. Nomenclature and structure-function relationships of transient receptor potential channels.Pharmacol. Rev. 57 (4): 427–50. 2. Müller D, Hoenderop JG, Meij IC, van den Heuvel LP, Knoers NV, den Hollander AI et al. (Nov 2000). Molecular cloning, tissue distribution, and chromosomal mapping of the human epithelial Ca2+ channel (ECAC1). Genomics 67 (1): 48–53. 3. Müller D, Hoenderop JG, Merkx GF, van Os CH, Bindels RJ (Sep 2000). Gene structure and chromosomal mapping of human epithelial calcium channel. Biochem. Biophys. Res. Commun. 275 (1): 47–52.

Uniprot ID: Q9NQA5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 83 kDa

Calculated Molecular Weight:

Gene ID: 56302

Protein Name: Transient receptor potential cation channel subfamily V member 5

Gene Full Name: transient receptor potential cation channel subfamily V member 5

Synonyms: Calcium transport protein 2; CAT2; ECaC; ECAC1; Epithelial calcium channel 1; Osm 9 like TRP channel 3; OTRPC3; TRPV5