Anti-TRIM24 Antibody Picoband® (monoclonal, 4G6C2)

SKU: M03258-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Gene Name: TRIM24

Specificity:

Background: Tripartite motif-containing 24 (TRIM24) also known as transcriptional intermediary factor 1α (TIF1α) is a protein that, in humans, is encoded by the TRIM24 gene. The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Immunogen: E.coli-derived human TRIM24 recombinant protein (Position: A706-D964).

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Beckstead, R., Ortiz, J. A., Sanchez, C., Prokopenko, S. N., Chambon, P., Losson, R., Bellen, H. J. Bonus, a Drosophila homolog of TIF1 proteins, interacts with nuclear receptors and can inhibit beta-FTZ-F1-dependent transcription. Molec. Cell 7: 753-765, 2001. 2. Ignat, M., Teletin, M., Tisserand, J., Khetchoumian, K., Dennefeld, C., Chambon, P., Losson, R., Mark, M. Arterial calcifications and increased expression of vitamin D receptor targets in mice lacking TIF1-alpha. Proc. Nat. Acad. Sci. 105: 2598-2603, 2008. 3. Khetchoumian, K., Teletin, M., Tisserand, J., Mark, M., Herquel, B., Ignat, M., Zucman-Rossi, J., Cammas, F., Lerouge, T., Thibault, C., Metzger, D., Chambon, P., Losson, R. Loss of Trim24 (Tif1-alpha) gene function confers oncogenic activity to retinoic acid receptor alpha. Nature Genet. 39: 1500-1506, 2007.

Uniprot ID: O15164

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: 4G6C2

Observed Molecular Weight: 130 kDa

Calculated Molecular Weight:

Gene ID: 8805

Protein Name: Transcription intermediary factor 1-alpha

Gene Full Name: tripartite motif containing 24

Synonyms: hTIF1; PTC6; RING finger protein 82; RNF82; TF1A; TIF1; TIF1 alpha; TIF1A; TIF1ALPHA; TRIM24; tripartite motif containing 24