Anti-TRBP/TARBP2 Antibody Picoband®

SKU: A01680-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: TARBP2

Specificity:

Background: RISC-loading complex subunit TARBP2 is a protein that in humans is encoded by the TARBP2 gene. HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene.

Immunogen: E.coli-derived human TRBP/TARBP2 recombinant protein (Position: E20-K366).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chendrimada, T. P., Gregory, R. I., Kumaraswamy, E., Norman, J., Cooch, N., Nishikura, K., Shiekhattar, R. TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing. (Letter) Nature 436: 740-744, 2005. 2. Garre, P., Perez-Segura, P., Diaz-Rubio, E., Caldes, T., de la Hoya, M. Reassessing the TARBP2 mutation rate in hereditary nonpolyposis colorectal cancer. (Letter) Nature Genet. 42: 817-818, 2010. 3. Gatignol, A., Buckler-White, A., Berkhout, B., Jeang, K. T. Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR. Science 251: 1597-1600, 1991.

Uniprot ID: Q15633

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 44 kDa

Calculated Molecular Weight: 50514 MW

Gene ID: 6895

Protein Name: RISC-loading complex subunit TARBP2

Gene Full Name: TARBP2 subunit of RISC loading complex

Synonyms: LOQS; TAR RNA binding protein 2; TARBP2; TRBP; TRBP1; TRBP2