Anti-TPX2 Antibody Picoband®

SKU: A01610-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ELISA

Application Details: Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml

Gene Name: TPX2

Specificity: No cross reactivity with other proteins.

Background: Targeting protein for Xklp2, also known as C20ORF1, is a protein that in humans is encoded by the TPX2 gene. It is mapped to chromosome 20q11.2. It was concluded that even in the presence of duplicated centrosomes, spindle formation requires the function of TPX2 to generate a stable bipolar spindle with overlapping antiparallel microtubule arrays. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.

Immunogen: E. coli-derived human TPX2 recombinant protein (Position: D15-Q93).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Gruss, O. J., Wittmann, M., Yokoyama, H., Pepperkok, R., Kufer, T., Sillje, H., Karsenti, E., Mattaj, I. W., Vernos, I. Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells. Nature Cell. Biol. 4: 871-879, 2002. 2. Kufer, T. A., Sillje, H. H. W., Korner, R., Gruss, O. J., Meraldi, P., Nigg, E. A. Human TPX2 is required for targeting Aurora-A kinase to the spindle. J. Cell Biol. 158: 617-623, 2002.

Uniprot ID: Q9ULW0

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 86 kDa, 100 kDa

Calculated Molecular Weight:

Gene ID: 22974

Protein Name: Targeting protein for Xklp2

Gene Full Name: TPX2 microtubule nucleation factor

Synonyms: C20orf1; C20orf2; DIL 2; DIL2; FLS353; GD:C20orf1; HCA519; HCTP4; p100; Protein fls353; REPP86; Targeting protein for Xklp2; TPX2