Anti-Topoisomerase I/TOP1 Antibody Picoband®

SKU: A00434-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IF,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
ELISA, 0.1-0.5μg/ml

Gene Name: TOP1

Specificity: No cross reactivity with other proteins.

Background: DNA topoisomerase 1 is an enzyme that in humans is encoded by the TOP1 gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

Immunogen: E. coli-derived human Topoisomerase I recombinant protein (Position: E526-F765).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: TOP1 topoisomerase (DNA) I. 2. Ahuja, H. G., Felix, C. A., Aplan, P. D. The t(11;20)(p15;q11) chromosomal translocation associated with therapy-related myelodysplastic syndrome results in an NUP98-TOP1fusion. Blood 94: 3258-3261, 1999.

Uniprot ID: P11387

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 100 kDa

Calculated Molecular Weight:

Gene ID: 7150

Protein Name: DNA topoisomerase 1

Gene Full Name: DNA topoisomerase I

Synonyms: DNA topoisomerase 1; DNA topoisomerase I; TOP1; TOPI; Top-I; TOPO1; topoisomerase (DNA) I