Anti-TOP3B Antibody Picoband®

SKU: A10446-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: TOP3B

Specificity:

Background: DNA topoisomerase 3-beta-1 is an enzyme that in humans is encoded by the TOP3B gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

Immunogen: E.coli-derived human TOP3B recombinant protein (Position: E128-R824).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kaufman, C. S., Genovese, A., Butler, M. G. Deletion of TOP3B is associated with cognitive impairment and facial dysmorphism. Cytogenet. Genome Res. 150: 106-111, 2016. 2. Kawasaki, K., Minoshima, S., Nakato, E., Shibuya, K., Shintani, A., Schmeits, J. L., Wang, J., Shimizu, N. One-megabase sequence analysis of the human immunoglobulin gamma gene locus. Genome Res. 7: 250-261, 1997. 3. Kwan, K. Y., Moens, P. B., Wang, J. C. Infertility and aneuploidy in mice lacking a type IA DNA topoisomerase III-beta. Proc. Nat. Acad. Sci. 100: 2526-2531, 2003.

Uniprot ID: O95985

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 90-97 kDa

Calculated Molecular Weight: 41409 MW

Gene ID: 8940

Protein Name: DNA topoisomerase 3-beta-1

Gene Full Name: DNA topoisomerase III beta

Synonyms: TOP3B; TOP3B1; DNA topoisomerase 3-beta-1; DNA topoisomerase III beta-1; DNA topoisomerase III beta