Anti-TNIK Antibody Picoband®

SKU: A03934-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: TNIK

Specificity:

Background: TRAF2 and NCK-interacting protein kinase is an enzyme that in humans is encoded by the TNIK gene. Wnt signaling plays important roles in carcinogenesis and embryonic development. The protein encoded by this gene is a serine/threonine kinase that functions as an activator of the Wnt signaling pathway. Mutations in this gene are associated with an autosomal recessive form of cognitive disability. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen: E.coli-derived human TNIK recombinant protein (Position: H553-E886).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Anazi, S., Shamseldin, H. E., AlNaqeb, D., Abouelhoda, M., Monies, D., Salih, M. A., Al-Rubeaan, K., Alkuraya, F. S. A null mutation in TNIK defines a novel locus for intellectual disability. Hum. Genet. 135: 773-778, 2016. 2. Coba, M. P., Komiyama, N. H., Nithianantharajah, J., Kopanitsa, M. V., Indersmitten, T., Skene, N. G., Tuck, E. J., Fricker, D. G., Elsegood, K. A., Stanford, L. E., Afinowi, N. O., Saksida, L. M., Bussey, T. J., O'Dell, T. J., Grant, S. G. N. TNiK is required for postsynaptic and nuclear signaling pathways and cognitive function. J. Neurosci. 32: 13987-13999, 2012. 3. Fu, C. A., Shen, M., Huang, B. C. B., Lasaga, J., Payan, D. G., Luo, Y. TNIK, a novel member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and regulates the cytoskeleton. J. Biol. Chem. 274: 30729-30737, 1999.

Uniprot ID: Q9UKE5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 180,150 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 23043

Protein Name: TRAF2 and NCK-interacting protein kinase

Gene Full Name: TRAF2 and NCK interacting kinase

Synonyms: TNIK; KIAA0551