Anti-Synaptophysin Syp Antibody (Monoclonal, SVP-38)

SKU: MA1091

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Rat

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, rat
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, rat, -
Western blot, 0.5-1μg/ml, Human, rat

Gene Name: SYP

Specificity: No cross reactivity with other proteins.

Background: Synaptophysin (SYP) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells. Ozcelik et al. (1990) concluded that the gene has 7 exons distributed over about 20 kb. SYP mapped the SYP locus to Xp11.23-p11.22. Regionalization of the gene on the X chromosome was also done with hamster/human hybrid cells in which various portions of the human X chromosome were present.

Immunogen: Rat retina synaptosome.

Clonality: Monoclonal

Contents: Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Ascites

Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Reference: 1. McMahon, H. T.; Bolshakov, V. Y.; Janz, R.; Hammer, R. E.; Siegelbaum, S. A.; Sudhof, T. C. : Synaptophysin, a major synaptic vesicle protein, is not essential for neurotransmitter release. Proc. Nat. Acad. Sci. 93: 4760-4764, 1996. 2. Cremin, S. M.; Greer, W. L.; Bodok-Nutzati, R.; Schwartz, M.; Peacocke, M.; Siminovitch, K. A. : Linkage of Wiskott-Aldrich syndrome with three marker loci, DXS426, SYP and TFE3, which map to the Xp11.3-p11.22 region. Hum. Genet. 92: 250-253, 1993. 3. Ozcelik, T.; Lafreniere, R. G.; Archer, B. T., III; Johnston, P. A.; Willard, H. F.; Francke, U.; Sudhof, T. C. : Synaptophysin: structure of the human gene and assignment to the X chromosome in man and mouse. Am. J. Hum. Genet. 47: 551-561, 1990.

Uniprot ID: P07825

Host: Mouse

Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: SVP-38

Observed Molecular Weight: 60 kDa

Calculated Molecular Weight: 33311 MW

Gene ID: Human(6855), Mouse(20977), Rat(24804)

Protein Name: aldehyde dehydrogenase (NAD(+))

Gene Full Name: synaptophysin

Synonyms: MRXSYP; Major synaptic vesicle protein p38