Anti-Symplekin/SYMPK Antibody Picoband®

SKU: A05709-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SYMPK

Specificity:

Background: Symplekin is a protein that in humans is encoded by the SYMPK gene. This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.

Immunogen: E.coli-derived human Symplekin/SYMPK recombinant protein (Position: K384-N1092).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Alwazzan, M., Hamshere, M. G., Lennon, G. G., Brook, J. D. Six transcripts map within 200 kilobases of the myotonic dystrophy expanded repeat. Mammalian Genome 9: 485-487, 1998. 2. Keon, B. H., Schafer, S., Kuhn, C., Grund, C., Franke, W. W. Symplekin, a novel type of tight junction plaque protein. J. Cell Biol. 134: 1003-1018, 1996. 3. Kolev, N. G., Steitz, J. A. Symplekin and multiple other polyadenylation factors participate in 3-prime-end maturation of histone mRNAs. Genes Dev. 19: 2583-2592, 2005.

Uniprot ID: Q92797

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 150 kDa

Calculated Molecular Weight: 101272 MW

Gene ID: 8189

Protein Name: Symplekin

Gene Full Name: symplekin scaffold protein

Synonyms: SPK; SYM; SYMPK; symplekin