Anti-SUPT16H Antibody Picoband®

SKU: A05199-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC/IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: SUPT16H

Specificity:

Background: Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit.

Immunogen: E.coli-derived human SUPT16H recombinant protein (Position: M1-E926). Human SUPT16H shares 98.6% amino acid (aa) sequence identity with mouse SUPT16H.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Belotserkovskaya, R., Oh, S., Bondarenko, V. A., Orphanides, G., Studitsky, V. M., Reinberg, D. FACT facilitates transcription-dependent nucleosome alteration. Science 301: 1090-1093, 2003. 2. Bina, R., Matalon, D., Fregeau, B., Tarsitano, J. J., Aukrust, I., Houge, G., Bend, R., Warren, H., Stevenson, R. E., Stuurman, K. E., Barkovich, A. J., Sherr, E. H. De novo variants in SUPT16H cause neurodevelopmental disorders associated with corpus callosum abnormalities. J. Med. Genet. 57: 461-465, 2020. 3. Keller, D. M., Zeng, X., Wang, Y., Zhang, Q. H., Kapoor, M., Shu, H., Goodman, R., Lozano, G., Zhao, Y., Lu, H. A DNA damage-induced p53 serine 392 kinase complex contains CK2, hSpt16, and SSRP1. Molec. Cell 7: 283-292, 2001.

Uniprot ID: Q9Y5B9

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 140 kDa

Calculated Molecular Weight: 120 kDa

Gene ID: 11198

Protein Name: FACT complex subunit SPT16

Gene Full Name: SPT16 homolog, facilitates chromatin remodeling subunit

Synonyms: SUPT16H; FACT140; FACTP140; FACT complex subunit SPT16; Chromatin-specific transcription elongation factor 140 kDa subunit; FACT 140 kDa subunit; FACTp140; Facilitates chromatin transcription complex subunit SPT16; hSPT16