Anti-SPRY3 Antibody Picoband®

SKU: A12965-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: SPRY3

Specificity:

Background: Involved in negative regulation of MAPK cascade. Predicted to be located in cytoplasm and membrane. Predicted to be active in cytosol.

Immunogen: E.coli-derived human SPRY3 recombinant protein (Position: M1-R162). Human SPRY3 shares 91.4% amino acid (aa) sequence identity with mouse SPRY3.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. De Bonis, M. L., Cerase, A., Matarazzo, M. R., Ferraro, M., Strazzullo, M., Hansen, R. S., Chiurazzi, P., Neri, G., D'Esposito, M. Maintenance of X- and Y-inactivation of the pseudoautosomal (PAR2) gene SPRY3 is independent from DNA methylation and associated to multiple layers of epigenetic modifications. Hum. Molec. Genet. 15: 1123-1132, 2006. 2. Hacohen, N., Kramer, S., Sutherland, D., Hiromi, Y., Krasnow, M. A. Sprouty encodes a novel antagonist of FGF signaling that patterns apical branching of the Drosophila airways. Cell 92: 253-263, 1998. 3. Ross, M. T., Grafham, D. V., Coffey, A. J., Scherer, S., McLay, K., Muzny, D., Platzer, M., Howell, G. R., Burrows, C., Bird, C. P., Frankish, A., Lovell, F. L., and 270 others. The DNA sequence of the human X chromosome. Nature 434: 325-337, 2005.

Uniprot ID: O43610

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 36 kDa

Calculated Molecular Weight: 31 kDa

Gene ID: 10251

Protein Name: Protein sprouty homolog 3

Gene Full Name: sprouty RTK signaling antagonist 3

Synonyms: HSPRY3, Protein sprouty homolog 3, sprouty homolog 3 (Drosophila), Spry 3, SPRY3