Anti-Spindly/SPDL1 Antibody Picoband®

SKU: A12722-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SPDL1

Specificity:

Background: This gene encodes a coiled-coil domain-containing protein that functions in mitotic spindle formation and chromosome segregation. The encoded protein plays a role in coordinating microtubule attachment by promoting recruitment of dynein proteins, and in mitotic checkpoint signaling.

Immunogen: E.coli-derived human Spindly/SPDL1 recombinant protein (Position: Q40-Q605).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Barisic, M., Sohm, B., Mikolcevic, P., Wandke, C., Rauch, V., Ringer, T., Hess, M., Bonn, G., Geley, S. Spindly/CCDC99 is required for efficient chromosome congression and mitotic checkpoint regulation. Molec. Biol. Cell 21: 1968-1981, 2010. 2. Chan, Y. W., Fava, L. L., Uldschmid, A., Schmitz, M. H. A., Gerlich, D. W., Nigg, E. A., Santamaria, A. Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly. J. Cell. Biol. 185: 859-874, 2009. 3. Griffis, E. R., Stuurman, N., Vale, R. D. Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore. J. Cell. Biol. 177: 1005-1015, 2007.

Uniprot ID: Q96EA4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 70 kDa

Calculated Molecular Weight:

Gene ID: 54908

Protein Name: Protein Spindly

Gene Full Name: spindle apparatus coiled-coil protein 1

Synonyms: CCDC99; hSpindly; MU-RMS-40.4A; Protein Spindly; spdly