Anti-SPATA18 Antibody Picoband®

SKU: A09906-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SPATA18

Specificity:

Background: This gene encodes a p53-inducible protein that is able to induce lysosome-like organelles within mitochondria that eliminate oxidized mitochondrial proteins, thereby contributing to mitochondrial quality control. Dysregulation of mitochondrial quality control is associated with cancer and degenerative diseases. The encoded protein mediates accumulation of the lysosome-like mitochondrial organelles through interaction with B cell lymphoma 2 interacting protein 3 and B cell lymphoma 2 interacting protein 3 like at the outer mitochondrial membrane, which allows translocation of lysosomal proteins to the mitochondrial matrix from the cytosol. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human SPATA18 recombinant protein (Position: E27-R490).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bornstein, C., Brosh, R., Molchadsky, A., Madar, S., Kogan-Sakin, I., Goldstein, I., Chakravarti, D., Flores, E. R., Goldfinger, N., Sarig, R., Rotter, V. SPATA18, a spermatogenesis-associated gene, is a novel transcriptional target of p53 and p63. Molec. Cell. Biol. 31: 1679-1689, 2011. 2. Iida, H., Ichinose, J., Kaneko, T., Mori, T., Shibata, Y. Complementary DNA cloning of rat Spetex-1, a spermatid-expressing gene-1, encoding a 63 kDa cytoplasmic protein of elongate spermatids. Molec. Reprod. Dev. 68: 385-393, 2004. 3. Kaindl, A. M., Jakubiczka, S., Lucke, T., Bartsch, O., Weis, J., Stoltenburg-Didinger, G., Aksu, F., Oexle, K., Koehler, K., Huebner, A. Homozygous microdeletion of chromosome 4q11-q12 causes severe limb-girdle muscular dystrophy type 2E with joint hyperlaxity and contractures. (Abstract) Hum. Mutat. 26: 279-280, 2005. Note: Full article online.

Uniprot ID: Q8TC71

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 61 kDa

Calculated Molecular Weight:

Gene ID: 132671

Protein Name: Mitochondria-eating protein

Gene Full Name: spermatogenesis associated 18

Synonyms: SPATA18; MIEAP; Mitochondria-eating protein; Spermatogenesis-associated protein 18