Anti-SMURF1 Antibody Picoband®

SKU: PB9892

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Rat

Applications: Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: SMURF1

Specificity: No cross reactivity with other proteins.

Background: E3 ubiquitin-protein ligase SMURF1 is an enzyme that in humans is encoded by the SMURF1 gene. It is mapped to 7q22.1. This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SMURF1, identical to the related mouse sequence.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: SMURF1 SMAD specific E3 ubiquitin protein ligase 1. 2. Yamaguchi K, Ohara O, Ando A, Nagase T (Apr 2008). Smurf1 ly targets hPEM-2, a GEF for Cdc42, via a novel combination of protein interaction modules in the ubiquitin-proteasome pathway. Biol. Chem. 389 (4): 405–13. 3. Zhu H, Kavsak P, Abdollah S, Wrana JL, Thomsen GH (Sep 1999). A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation. Nature 400 (6745): 687–93.

Uniprot ID: Q9HCE7

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 86 kDa

Calculated Molecular Weight: 86114 MW

Gene ID: 57154

Protein Name: E3 ubiquitin-protein ligase SMURF1

Gene Full Name: SMAD specific E3 ubiquitin protein ligase 1

Synonyms: hSMURF1; KIAA1625; SMURF1