Anti-SMAP2 Antibody Picoband®

SKU: A07564-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IP,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: SMAP2

Specificity:

Background: Predicted to enable GTPase activator activity. Located in cytosol and nucleoplasm.

Immunogen: E.coli-derived human SMAP2 recombinant protein (Position: K142-K429). Human SMAP2 shares 94.1% amino acid (aa) sequence identity with mouse SMAP2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Hartz, P. A. Personal Communication. Baltimore, Md. 4/22/2016. 2. Kobayashi, N., Kon, S., Henmi, Y., Funaki, T., Satake, M., Tanabe, K. The Arf GTPase-activating protein SMAP1 promotes transferrin receptor endocytosis and interacts with SMAP2. Biochem. Biophys. Res. Commun. 453: 473-479, 2014. 3. Natsume, W., Tanabe, K., Kon, S., Yoshida, N., Watanabe, T., Torii, T., Satake, M. SMAP2, a novel ARF GTPase-activating protein, interacts with clathrin and clathrin assembly protein and functions on the AP-1-positive early endosome/trans-Golgi network. Molec. Biol. Cell 17: 2592-2603, 2006.

Uniprot ID: Q8WU79

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 47 kDa

Calculated Molecular Weight: 47 kDa

Gene ID: 64744

Protein Name: Stromal membrane-associated protein 2

Gene Full Name: small ArfGAP2

Synonyms: RP1 228H13.3; small ArfGAP2; SMAP1L; SMAP2