Anti-Smad9 Antibody Picoband®

SKU: A04932-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: Smad9

Specificity:

Background: Mothers against decapentaplegic homolog 9 also known as SMAD9, SMAD8, and MADH6 is a protein that in humans is encoded by the SMAD9 gene. This gene encodes a member of a family of proteins that act as downstream effectors of the bone morphogenetic protein (BMP) signaling pathway. The encoded protein is phosphorylated by BMP receptors, which stimulates its binding to SMAD4 and translocation into the nucleus, where it functions as a regulator of transcription. Activity of this protein is important for embryonic development. Mutation of this gene results in defects in pulmonary vasculature.

Immunogen: E.coli-derived mouse Smad9 recombinant protein (Position: Q153-H235). Mouse Smad9 shares 59.2% and 96.4% amino acid (aa) sequence identity with human and rat Smad9, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chen, Y., Bhushan, A., Vale, W. Smad8 mediates the signaling of the ALK-2 receptor serine kinase. Proc. Nat. Acad. Sci. 94: 12938-12943, 1997. Note: Erratum: Proc. Nat. Acad. Sci. 95: 1968 only, 1998. 2. Davis, B. N., Hilyard, A. C., Lagna, G., Hata, A. SMAD proteins control DROSHA-mediated microRNA maturation. Nature 454: 56-61, 2008. 3. Drake, K. M., Zygmunt, D., Mavrakis, L., Harbor, P., Wang, L., Comhair, S. A., Erzurum, S. C., Aldred, M. A. Altered microRNA processing in heritable pulmonary arterial hypertension: an important role for Smad-8. Am. J. Resp. Crit. Care Med. 184: 1400-1408, 2011.

Uniprot ID: Q9JIW5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 55 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 55994

Protein Name: Mothers against decapentaplegic homolog 9

Gene Full Name: SMAD family member 9

Synonyms: MAD homolog 9; MADH6; MADH9; Mothers against DPP homolog 9; SMAD 9; SMAD family member 9; SMAD8A; SMAD8B; SMAD9