Anti-SKA2 Antibody Picoband®

SKU: A06326

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: Flow Cytometry,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: SKA2

Specificity: No cross reactivity with other proteins.

Background: Spindle and kinetochore-associated protein 2 is a protein that in humans is encoded by the SKA2 gene found in chromosome 17.SKA2 is a part of a spindle and kinetochore associated complex also including SKA1 and SKA3 which is responsible for onset of the anaphase in mitosis by regulating chromosomal segregation. SKA2 may function as a prognostic gene marker for identifying lung cancer[3] as well as a proposed biomarker for suicidal tendencies and post-traumatic stress disorders.[4][5] The SKA2 gene contains one single-nucleotide polymorphism (SNP) rs7208505 located in the 3' UTR. This genetic variant containing a cytosine (existing in the less common allele) instead of thymine along with epigenetic modification (such as DNA methylation) is correlated with suicidal tendencies and post-traumatic stress

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SKA2, which shares 90.0% amino acid (aa) sequence identity with both mouse and rat SKA2.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Hanisch A, Silljé HH, Nigg EA (November 2006). Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska2. The EMBO Journal. 25 (23): 5504–15. 2. Wang Y, Zhang Y, Zhang C, Weng H, Li Y, Cai W, Xie M, Long Y, Ai Q, Liu Z, Du G, Wang S, Niu Y, Song F, Ozaki T, Bu Y (September 2015). The gene pair PRR11 and SKA2 shares a NF-Y-regulated biional promoter and contributes to lung cancer development. Biochimica et Biophysica Acta. 1849 (9): 1133–44. 3. Guintivano J, Brown T, Newcomer A, Jones M, Cox O, Maher BS, Eaton WW, Payne JL, Wilcox HC, Kaminsky ZA (December 2014). Identification and replication of a combined epigenetic and genetic biomarker predicting suicide and suicidal behaviors. The American Journal of Psychiatry. 171 (12): 1287–96.

Uniprot ID: Q8WVK7

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 20 kDa

Calculated Molecular Weight: 39162 MW

Gene ID: 348235

Protein Name: Spindle and kinetochore-associated protein 2

Gene Full Name: spindle and kinetochore associated complex subunit 2

Synonyms: spindle and kinetochore associated complex subunit 2; FAM33A; SKA2