Anti-SF3A1 Antibody Picoband®

SKU: A05483-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,IP,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human, Rat
Immunoprecipitation, 0.5-2 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SF3A1

Specificity:

Background: Splicing factor 3 subunit 1 is a protein that in humans is encoded by the SF3A1 gene. This gene encodes a subunit of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP). U2 small nuclear ribonucleoproteins play a critical role in spliceosome assembly and pre-mRNA splicing.

Immunogen: E.coli-derived human SF3A1 recombinant protein (Position: K20-Q556).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Isono, K., Abe, K., Tomaru, Y., Okazaki, Y., Hayashizaki, Y., Koseki, H. Molecular cloning, genetic mapping, and expression of the mouse Sf3b1 (SAP155) gene for the U2 snRNP component of spliceosome. Mammalian Genome 12: 192-198, 2001. 2. Kramer, A., Mulhauser, F., Wersig, C., Groning, K., Bilbe, G. Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of Saccharomyces cerevisiae. RNA 1: 260-272, 1995. 3. Wang, C., Chua, K., Seghezzi, W., Lees, E., Gozani, O., Reed, R. Phosphorylation of spliceosomal protein SAP 155 coupled with splicing catalysis. Genes Dev. 12: 1409-1414, 1998.

Uniprot ID: Q15459

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 120-130 kDa

Calculated Molecular Weight: 48608 MW

Gene ID: 10291

Protein Name: Splicing factor 3A subunit 1

Gene Full Name: splicing factor 3a subunit 1

Synonyms: PRP21; PRPF21; SAP 114; SAP114; SF3A1; SF3A120; Splicing factor 3A subunit 1