Anti-Semaphorin 7a/SEMA7A Antibody Picoband®

SKU: A03832-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SEMA7A

Specificity:

Background: Semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) (SEMA7A) also known as CD108 (Cluster of Differentiation 108), is a human gene. This gene encodes a member of the semaphorin family of proteins. The encoded preproprotein is proteolytically processed to generate the mature glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein. The encoded protein is found on activated lymphocytes and erythrocytes and may be involved in immunomodulatory and neuronal processes. The encoded protein carries the John Milton Hagen (JMH) blood group antigens. Mutations in this gene may be associated with reduced bone mineral density (BMD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Immunogen: E.coli-derived human Semaphorin 7a/SEMA7A recombinant protein (Position: E273-H572).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Czopik, A. K., Bynoe, M. S., Palm, N., Raine, C. S., Medzhitov, R. Semaphorin 7A is a negative regulator of T cell responses. Immunity 24: 591-600, 2006. 2. Gross, M. B. Personal Communication. Baltimore, Md. 9/13/2017. 3. Koh, J.-M., Oh, B., Lee, J. Y., Lee, J.-K., Kimm, K., Kim, G. S., Park, B. L., Cheong, H. S., Shin, H. D., Hong, J. M., Kim, T.-H., Park, E. K., Kim, S.-Y. Association study of semaphorin 7a (sema7a) polymorphisms with bone mineral density and fracture risk in postmenopausal Korean women. J. Hum. Genet. 51: 112-117, 2006.

Uniprot ID: O75326

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 75 kDa

Calculated Molecular Weight: 127459 MW

Gene ID: 8482

Protein Name: Semaphorin-7A

Gene Full Name: semaphorin 7A (JohnMiltonHagen blood group)

Synonyms: SEMA7A; CD108; SEMAL; Semaphorin-7A; CDw108; JMH blood group antigen; Semaphorin-K1; Sema K1; Semaphorin-L; Sema L; CD antigen CD108; H SEMA K1; H Sema L; JMH; Sema K1; SEMAK1; Semaphorin 7A; Semaphorin K1; Semaphorin L