Anti-SECISBP2 Antibody Picoband®

SKU: A06543-4

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SECISBP2

Specificity:

Background: SECIS-binding protein 2 (commonly referred to as SBP2) is a protein that in humans is encoded by the SECISBP2 gene. The protein encoded by this gene is one of the essential components of the machinery involved in co-translational insertion of selenocysteine (Sec) into selenoproteins. Sec is encoded by the UGA codon, which normally signals translation termination. The recoding of UGA as Sec codon requires a Sec insertion sequence (SECIS) element; present in the 3' untranslated regions of eukaryotic selenoprotein mRNAs. This protein specifically binds to the SECIS element, which is stimulated by a Sec-specific translation elongation factor. Mutations in this gene have been associated with reduction in enzymatic activity of type II iodothyronine deiodinase (a selenoprotein) and abnormal thyroid hormone metabolism. Alternatively spliced transcript variants have been found for this gene.

Immunogen: E.coli-derived human SECISBP2 recombinant protein (Position: E12-L854). Human SECISBP2 shares 71.7% amino acid (aa) sequence identity with rat SECISBP2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Catli, G., Fujisawa, H., Kirbiyik, O., Mimoto, M. S., Gencpinar, P., Ozdemir, T. R., Dundar, B. N., Dumitrescu, A. M. A novel homozygous selenocysteine insertion sequence binding protein 2 (SECISBP2, SBP2) gene mutation in a Turkish boy. Thyroid 28: 1221-1223, 2018. 2. Copeland, P. R., Fletcher, J. E., Carlson, B. A., Hatfield, D. L., Driscoll, D. M. A novel RNA binding protein, SBP2, is required for the translation of mammalian selenoprotein mRNAs. EMBO J. 19: 306-314, 2000. 3. Dumitrescu, A. M., Liao, X.-H., Abdullah, M. S. Y., Lado-Abeal, J., Majed, F. A., Moeller, L. C., Boran, G., Schomburg, L., Weiss, R. E., Refetoff, S. Mutations in SECISBP2 result in abnormal thyroid hormone metabolism. Nature Genet. 37: 1247-1252, 2005.

Uniprot ID: Q96T21

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 105 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 79048

Protein Name: Selenocysteine insertion sequence-binding protein 2

Gene Full Name: SECIS binding protein 2

Synonyms: SBP2; SECIS binding protein 2; SECISBP2