Anti-SCYL2 Antibody Picoband®

SKU: A08578-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: SCYL2

Specificity:

Background: SCY1-like protein 2 is a protein that in humans is encoded by the SCYL2 gene. The protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene.

Immunogen: E.coli-derived human SCYL2 recombinant protein (Position: E208-Q909).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Conner, S. D., Schmid, S. L. CVAK104 is a novel poly(L)-lysine-stimulated kinase that targets the beta-2-subunit of AP2. J. Biol. Chem. 280: 21539-21544, 2005. 2. Gingras, S., Earls, L. R., Howell, S., Smeyne, R. J., Zakharenko, S. S., Pelletier, S. SCYL2 protects CA3 pyramidal neurons from excitotoxicity during functional maturation of the mouse hippocampus. J. Neurosci. 35: 10510-10522, 2015. 3. Gross, M. B. Personal Communication. Baltimore, Md. 5/15/2015.

Uniprot ID: Q6P3W7

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 104 kDa

Calculated Molecular Weight: 45470 MW

Gene ID: 55681

Protein Name: SCY1-like protein 2

Gene Full Name: SCY1 like pseudokinase 2

Synonyms: CVAK104; KIAA1360; SCY1 like 2 (S. cerevisiae); SCY1 like protein 2; SCYL2