Anti-SA1/STAG1 Antibody Picoband®

SKU: A06471-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: STAG1

Specificity:

Background: Cohesin subunit SA-1 (SA1) is a protein that in humans is encoded by the STAG1 gene. This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.

Immunogen: E.coli-derived human SA1/STAG1 recombinant protein (Position: Q748-L1224).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Carramolino, L., Lee, B. C., Zaballos. A., Peled, A., Barthelemy, I., Shav-Tal, Y., Prieto, I., Carmi, P., Gothelf, Y., Gonzalez de Buitrago, G., Aracil, M., Marquez, G., Barbero, J. L., Zipori, D. SA-1, a nuclear protein encoded by one member of a novel gene family: molecular cloning and detection in hemopoietic organs. Gene 195: 151-159, 1997. Note: Erratum: Gene 206: 283 only, 1998. 2. Davidson, I. F., Bauer, B., Goetz, D., Tang, W., Wutz, G., Peters, J. M. DNA loop extrusion by human cohesin. Science 366: 1338-1345, 2019. 3. Lehalle, D., Mosca-Boidron, A.-L., Begtrup, A., Boute-Benejean, O., Charles, P., Cho, M. T., Clarkson, A., Devinsky, O., Duffourd, Y., Duplomb-Jego, L., Gerard, B., Jacquette, A., and 25 others. STAG1 mutations cause a novel cohesinopathy characterised by unspecific syndromic intellectual disability. J. Med. Genet. 54: 479-488, 2017.

Uniprot ID: Q8WVM7

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 155 kDa

Calculated Molecular Weight:

Gene ID: 10274

Protein Name: Cohesin subunit SA-1

Gene Full Name: STAG1 cohesin complex component

Synonyms: Cohesin subunit SA 1; SA1; SCC3 homolog 1; STAG1; stromal antigen 1