Anti-RYK Antibody Picoband®

SKU: A07225-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: RYK

Specificity:

Background: The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Immunogen: E.coli-derived human RYK recombinant protein (Position: L66-V607). Human RYK shares 97.2% amino acid (aa) sequence identity with mouse RYK.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Gough, N. M., Rakar, S., Hovens, C. M., Wilks, A. Localization of two mouse genes encoding the protein tyrosine kinase receptor-related protein RYK. Mammalian Genome 6: 255-256, 1995. 2. Halford, M. M., Armes, J., Buchert, M., Meskenaite, V., Grail, D., Hibbs, M. L., Wilks, A. F., Farlie, P. G., Newgreen, D. F., Hovens, C. M., Stacker, S. A. Ryk-deficient mice exhibit craniofacial defects associated with perturbed Eph receptor crosstalk. Nature Genet. 25: 414-418, 2000. 3. Hovens, C. M., Stacker, S. A., Andres, A.-C., Harpur, A. G., Wilks, A. F. RYK, a receptor tyrosine kinase-related molecule with unusual kinase domain motifs. Proc. Nat. Acad. Sci. 89: 11818-11822, 1992.

Uniprot ID: P34925

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 75 kDa

Calculated Molecular Weight: 68 kDa

Gene ID: 6259

Protein Name: Tyrosine-protein kinase RYK

Gene Full Name: receptor like tyrosine kinase

Synonyms: D3S3195; JTK5; JTK5A; RYK; RYK1; Tyrosine protein kinase RYK