Anti-RPN2 Antibody Picoband®

SKU: A05006-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IF,ICC,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RPN2

Specificity:

Background: Dolichyl-diphosphooligosaccharide—protein glycosyltransferase subunit 2, also called ribophorin ∥ is an enzyme that in humans is encoded by the RPN2 gene. This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human RPN2 recombinant protein (Position: Q64-Q174).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Barton, D. E., Crimaudo, C., Hortsch, M., Francke, U. The genes for ribophorins I and II are on human chromosomes 3q and 20 and mouse chromosomes 6 and 12, respectively. (Abstract) Cytogenet. Cell Genet. 46: 577 only, 1987. 2. Crimaudo, C., Hortsch, M., Gausepohl, H., Meyer, D. I. Human ribophorins I and II: the primary structure and membrane topology of two highly conserved round endoplasmic reticulum-specific glycoproteins. EMBO J. 6: 75-82, 1987. 3. Honma, K., Iwao-Koizumi, K., Takeshita, F., Yamamoto, Y., Yoshida, T., Nishio, K., Nagahara, S., Kato, K., Ochiya, T. RPN2 gene confers docetaxel resistance in breast cancer. Nature Med. 14: 939-948, 2008. Note: Erratum: Nature Med. 14: 1128 only, 2008.

Uniprot ID: P04844

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 69 kDa

Calculated Molecular Weight: 25847 MW

Gene ID: 6185

Protein Name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2

Gene Full Name: ribophorin II

Synonyms: RIBIIR; Ribophorin 2; ribophorin II; RPN II; RPN2; RPNII; SWP1