Anti-RANTES/CCL5 Antibody Picoband®

SKU: PB9573

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, -
ELISA, 0.1-0.5μg/ml, -

Gene Name: CCL5

Specificity: No cross reactivity with other proteins.

Background: Chemokine (C-C motif) ligand 5 (also CCL5) is a protein which in humans is encoded by the CCL5 gene. It is also known as RANTES. This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms.

Immunogen: E. coli-derived human RANTES recombinant protein (Position: Y26-S91). Human RANTES shares 83.3% amino acid (aa) sequence identity with both mouse and rat RANTES.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Donlon TA, Krensky AM, Wallace MR, Collins FS, Lovett M, Clayberger C (March 1990). Localization of a human T-cell-specific gene, RANTES (D17S136E), to chromosome 17q11.2-q12. Genomics 6 (3): 548–53. 2. Maghazachi AA, Al-Aoukaty A, Schall TJ (February 1996). CC chemokines induce the generation of killer cells from CD56+ cells. Eur. J. Immunol. 26 (2): 315–9. 3. Song A, Nikolcheva T, Krensky AM (October 2000). Transcriptional regulation of RANTES expression in T lymphocytes. Immunol. Rev. 177: 236–45.

Uniprot ID: P13501

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 11 kDa

Calculated Molecular Weight: 9990 MW

Gene ID: 6352

Protein Name: C-C motif chemokine 5

Gene Full Name: C-C motif chemokine ligand 5

Synonyms: C C motif chemokine 5; CCL5; chemokine (C C motif) ligand 5; D17S136E; EoCP; RANTES; SCYA5; SIS delta; SISd; Small inducible cytokine A5; T cell specific protein P228; T cell specific protein RANTES; TCP228