Anti-RANKL/TNFSF11 Antibody Picoband®

SKU: PB10015

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: ELISA,WB

Application Details: ELISA, 0.1-0.5μg/ml, -, Rat
Western blot, 0.1-0.5μg/ml, Rat

Gene Name: Tnfsf11

Specificity: No cross reactivity with other proteins.

Background: Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. It is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

Immunogen: E. coli-derived rat RANKL recombinant protein (Position: F141-D318). Rat RANKL shares 87.9% and 96.6% amino acid (aa) sequence identity with human and mouse RANKL, respectively.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Asselin-Labat, M.-L., Vaillant, F., Sheridan, J. M., Pal, B., Wu, D., Simpson, E. R. Yasuda, H.; Smyth, G. K.; Martin, T. J.; Lindeman, G. J.; Visvader, J. E.: Control of mammary stem cell function by steroid hormone signalling. Nature 465: 798-802, 2010. 2. Luan, X., Lu, Q., Jiang, Y., Zhang, S., Wang, Q., Yuan, H., Zhao, W., Wang, J., Wang, X.Crystal structure of human RANKL complexed with its decoy receptor osteoprotegerin. J Immun. 189: 245-252, 2012.

Uniprot ID: Q9ESE2

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 40 kDa

Calculated Molecular Weight: 35370 MW

Gene ID: 117516

Protein Name: Tumor necrosis factor ligand superfamily member 11

Gene Full Name: TNF superfamily member 11

Synonyms: CD254; hRANKL2; ODF; OPGL; OPTB2; Osteoprotegerin ligand; RANKL; sOdf; TNFSF11; TRANCE