Anti-RANGAP1 Antibody Picoband®

SKU: A02771-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: RANGAP1

Specificity:

Background: Ran GTPase-activating protein 1 is an enzyme that in humans is encoded by the RANGAP1 gene. This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human RANGAP1 recombinant protein (Position: A165-V587).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bernier-Villamor, V., Sampson, D. A., Matunis, M. J., Lima, C. D. Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1. Cell 108: 345-356, 2002. 2. Bischoff, F. R., Klebe, C., Kretschmer, J., Wittinghofer, A., Ponstingl, H. RanGAP1 induces GTPase activity of nuclear Ras-related Ran. Proc. Nat. Acad. Sci. 91: 2587-2591, 1994. 3. Bischoff, F. R., Krebber, H., Kempf, T., Hermes, I., Ponstingl, H. Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport. Proc. Nat. Acad. Sci. 92: 1749-1753, 1995.

Uniprot ID: P46060

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 70 kDa

Calculated Molecular Weight: 12252 MW

Gene ID: 5905

Protein Name: Ran GTPase-activating protein 1

Gene Full Name: Ran GTPase activating protein 1

Synonyms: Fug1; KIAA1835; RANGAP1; SD