Anti-PUMA/BBC3 Antibody Picoband®

SKU: PA1313

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Gene Name: BBC3

Specificity: No cross reactivity with other proteins.

Background: The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91% amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PUMA, identical to the related rat and mouse sequences.

Clonality: Polyclonal

Contents: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Nakano K, Vousden KH (March 2001). PUMA, a novel proapoptotic gene, is induced by p53. Mol. Cell 7 (3): 683–94. 2. Han J, Flemington C, Houghton AB, Gu Z, Zambetti GP, Lutz RJ, Zhu L, Chittenden T (September 2001). Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. Proc. Natl. Acad. Sci. U.S.A. 98 (20): 11318–23. 3. Yu, J.; Zhang, L.; Hwang, P. M.; Kinzler, K. W.; Vogelstein, B. : PUMA induces the rapid apoptosis of colorectal cancer cells. Molec. Cell 7: 673-682, 2001. 4. Nakano, K.; Vousden, K. H. : PUMA, a novel proapoptotic gene, is induced by p53. Molec. Cell 7: 683-694, 2001. 5. Chipuk, J. E.; Bouchier-Hayes, L.; Kuwana, T.; Newmeyer, D. D.; Green, D. R. : PUMA couples the nuclear and cytoplasmic proapoptotic function of p53. Science 309: 1732-1735, 2005.

Uniprot ID: Q9BXH1

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 21 kDa

Calculated Molecular Weight: 20532 MW

Gene ID: 27113

Protein Name: Bcl-2-binding component 3, isoforms 1/2

Gene Full Name: BCL2 binding component 3

Synonyms: BBC3; JFY 1; JFY1; PUMA