Anti-Psmb1 Antibody Picoband®

SKU: A06891-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: HUman,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5μg/ml, -

Gene Name: Psmb1

Specificity:

Background: Proteasome subunit beta type-1 also known as 20S proteasome subunit beta-6 (based on systematic nomenclature) is a protein that in humans is encoded by the PSMB1 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is tightly linked to the TBP (TATA-binding protein) gene in human and in mouse, and is transcribed in the opposite orientation in both species.

Immunogen: E.coli-derived mouse Psmb1 recombinant protein (Position: S21-D240).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Okumura, K., Nogami, M., Taguchi, H., Hisamatsu, H., Tanaka, K. The genes for the alpha-type HC3 (PMSA2) and beta-type HC5 (PMSB1) subunits of human proteasomes map to chromosomes 6q27 and 7p12-p13 by fluorescence in situ hybridization. Genomics 27: 377-379, 1995. 2. Tamura, T., Osaka, F., Kawamura, Y., Higuti, T., Ishida, N., Nothwang, H.-G., Tsurumi, C., Tanaka, K., Ichihara, A. Isolation and characterization of alpha-type HC3 and beta-type HC5 subunit genes of human proteasomes. J. Molec. Biol. 244: 117-124, 1994. 3. Trachtulec, Z., Hamvas, R. M. J., Forejt, J., Lehrach, H. R., Vincek, V., Klein, J. Linkage of TATA-binding protein and proteasome subunit C5 genes in mice and humans reveals synteny conserved between mammals and invertebrates. Genomics 44: 1-7, 1997.

Uniprot ID: O09061

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 26 kDa

Calculated Molecular Weight: 87148 MW

Gene ID: 19170

Protein Name: Proteasome subunit beta type-1

Gene Full Name: proteasome (prosome, macropain) subunit, beta type 1

Synonyms: 20S proteasome; HC5; KIAA1838; Macropain subunit C5; PMSB1; Proteasome component C5; Proteasome gamma chain; Proteasome subunit beta type 1; PSC5; PSMB1