Anti-PRPF19 Antibody Picoband®

SKU: A02434-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PRPF19

Specificity:

Background: Pre-mRNA-processing factor 19 is a protein that in humans is encoded by the PRPF19 gene. Enables identical protein binding activity and ubiquitin-ubiquitin ligase activity. Involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome. Acts upstream of or within protein polyubiquitination. Located in cytoplasm; nuclear speck; and site of double-strand break. Part of Prp19 complex and U2-type catalytic step 2 spliceosome. Colocalizes with DNA replication factor A complex.

Immunogen: E.coli-derived human PRPF19 recombinant protein (Position: Q51-H359).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Beck, B. D., Park, S.-J., Lee, Y.-J., Roman, Y., Hromas, R. A., Lee, S.-H. Human Pso4 is a Metnase (SETMAR)-binding partner that regulates Metnase function in DNA repair. J. Biol. Chem. 283: 9023-9030, 2008. 2. Gross, M. B. Personal Communication. Baltimore, Md. 9/6/2016. 3. Mahajan, K. N., Mitchell, B. S. Role of human Pso4 in mammalian DNA repair and association with terminal deoxynucleotidyl transferase. Proc. Nat. Acad. Sci. 100: 10746-10751, 2003.

Uniprot ID: Q9UMS4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 54 kDa

Calculated Molecular Weight: 38779 MW

Gene ID: 27339

Protein Name: Pre-mRNA-processing factor 19

Gene Full Name: pre-mRNA processing factor 19

Synonyms: hPSO4; Nuclear matrix protein 200; Pre mRNA processing factor 19; PRP19; PRP19/PSO4 homolog; PRPF19; PSO4; Senescence evasion factor; SNEV; UBOX4