Anti-PRC1 Antibody Picoband®

SKU: PB9814

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IP,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: PRC1

Specificity: No cross reactivity with other proteins.

Background: Protein Regulator of cytokinesis 1 (PRC1) is a protein that in humans is encoded by the PRC1 gene and is involved in cytokinesis. This gene is mapped to chromosome 15q26.1 based on an alignment of the PRC1 sequence with the genomic sequence. PRC1 protein is expressed at relatively high levels during S and G2/M phases of the cell cycle before dropping dramatically after mitotic exit and entrance into G1 phase. It is located in the nucleus during interphase, becomes associated with the mitotic spindle in a highly dynamic manner during anaphase, and localizes to the cell midbody during cytokinesis. And this protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human PRC1 recombinant protein (Position: M1-E233). Human PRC1 shares 85% amino acid (aa) sequence identity with mouse PRC1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: PRC1 protein regulator of cytokinesis 1. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 2/1/2005. 3. Jiang W, Jimenez G, Wells NJ, Hope TJ, Wahl GM, Hunter T, Fukunaga R (1998). PRC1: a human mitotic spindle-associated CDK substrate protein required for cytokinesis.. Mol. Cell. 2 (6): 877–85.

Uniprot ID: O43663

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 72 kDa

Calculated Molecular Weight: 72 kDa

Gene ID: 9055

Protein Name: Protein regulator of cytokinesis 1

Gene Full Name: protein regulator of cytokinesis 1

Synonyms: ASE1; PRC1