Anti-PPAN Antibody Picoband®

SKU: A10836-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PPAN

Specificity:

Background: Suppressor of SWI4 1 homolog is a protein that in humans is encoded by the PPAN gene. The protein encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Read-through transcription of this gene with P2RY11/P2Y(11), an adjacent downstream gene that encodes an ATP receptor, has been found. These read-through transcripts are ubiquitously present and up-regulated during granulocyte differentiation.

Immunogen: E.coli-derived human PPAN recombinant protein (Position: R20-E288).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Communi, D., Suarez-Huerta, N., Dussossoy, D., Savi, P, Boeynaems, J.-M. Cotranscription and intergenic splicing of human P2Y11 and SSF1 genes. J. Biol. Chem. 276: 16561-16566, 2001. 2. Suarez-Huerta, N., Boeynaems, J.-M., Communi, D. Cloning, genomic organization, and tissue distribution of human Ssf-1. Biochem. Biophys. Res. Commun. 275: 37-42, 2000. 3. Welch, P. J., Marcusson, E. G., Li, Q.-X., Beger, C., Kruger, M., Zhou, C., Leavitt, M., Wong-Staal, F., Barber, J. R. Identification and validation of a gene involved in anchorage-independent cell growth control using a library of randomized hairpin ribozymes. Genomics 66: 274-283, 2000.

Uniprot ID: Q9NQ55

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 65 kDa

Calculated Molecular Weight: 29370 MW

Gene ID: 56342

Protein Name: Suppressor of SWI4 1 homolog

Gene Full Name: peter pan homolog

Synonyms: BXDC3; Peter Pan homolog; PPAN; SSF; Ssf 1; SSF1; SSF2; Suppressor of SWI4 1 homolog