Anti-POLR3F Antibody Picoband®

SKU: A12720-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: POLR3F

Specificity:

Background: DNA-ed RNA polymerase III subunit RPC6 is an enzyme that in humans is encoded by the POLR3F gene. The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human POLR3F recombinant protein (Position: K7-E312). Human POLR3F shares 98.7% amino acid (aa) sequence identity with mouse POLR3F.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Carter-Timofte, M. E., Hansen, A. F., Mardahl, M., Fribourg, S., Rapaport, F., Zhang, S.-Y., Casanova, J.-L., Paludan, S. R., Christiansen, M., Larsen, C. S., Mogensen, T. H. Varicella-zoster virus CNS vasculitis and RNA polymerase III gene mutation in identical twins. Neurol. Neuroimmun. Neuroinflamm. 5: e500, 2018. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 04/26/2017. 3. Wang, Z., Roeder, R. G. Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation. Genes Dev. 11: 1315-1326, 1997.

Uniprot ID: Q9H1D9

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 36 kDa

Calculated Molecular Weight:

Gene ID: 10621

Protein Name: DNA-directed RNA polymerase III subunit RPC6

Gene Full Name: RNA polymerase III subunit F

Synonyms: POLR3F; DNA-directed RNA polymerase III subunit RPC6; RNA polymerase III subunit C6; DNA-directed RNA polymerase III subunit F; RNA polymerase III 39 kDa subunit; RPC39