Anti-POLM Antibody Picoband®

SKU: A08812-2

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IP,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: POLM

Specificity:

Background: Predicted to enable DNA-directed DNA polymerase activity. Predicted to be involved in double-strand break repair via nonhomologous end joining. Predicted to act upstream of or within B cell differentiation and somatic hypermutation of immunoglobulin genes. Predicted to be located in nucleoplasm. Predicted to be active in nucleus.

Immunogen: E.coli-derived human POLM recombinant protein (Position: E36-H481). Human POLM shares 76.5% amino acid (aa) sequence identity with mouse POLM.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Aoufouchi, S., Flatter, E., Dahan, A., Faili, A., Bertocci, B., Storck, S., Delbos, F., Cocea, L., Gupta, N., Weill, J.-C., Reynaud, C.-A. Two novel human and mouse DNA polymerases of the polX family. Nucleic Acids Res. 28: 3684-3693, 2000. 2. Bertocci, B., De Smet, A., Berek, C., Weill, J.-C., Reynaud, C.-A. Immunoglobulin kappa light chain gene rearrangement is impaired in mice deficient for DNA polymerase mu. Immunity 19: 203-211, 2003. 3. Bertocci, B., De Smet, A., Flatter, E., Dahan, A., Bories, J.-C., Landreau, C., Weill, J.-C., Reynaud, C.-A. Cutting edge: DNA polymerase mu and lambda are dispensable for Ig gene hypermutation. J. Immun. 168: 3702-3706, 2002.

Uniprot ID: Q9NP87

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 55 kDa

Calculated Molecular Weight: 55 kDa

Gene ID: 27434

Protein Name: DNA-directed DNA/RNA polymerase mu

Gene Full Name: DNA polymerase mu

Synonyms: POLM; polmu; DNA-directed DNA/RNA polymerase mu; Pol Mu; EC 2.7.7.7; Terminal transferase