Anti-Pleckstrin/PLEK Antibody Picoband®

SKU: A06656-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PLEK

Specificity:

Background: Enables phosphatidylinositol-3,4-bisphosphate binding activity; protein homodimerization activity; and protein kinase C binding activity. Involved in several processes, including G protein-coupled receptor signaling pathway; actin cytoskeleton organization; and positive regulation of supramolecular fiber organization. Located in cytoplasm and ruffle membrane.

Immunogen: E.coli-derived human Pleckstrin/PLEK recombinant protein (Position: M47-K308).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Cmarik, J. L., Hegamyer, G., Gerrard, B., Dean, M., Colburn, N. H. cDNA cloning and mapping of mouse pleckstrin (Plek), a gene upregulated in transformation-resistant cells. Genomics 66: 204-212, 2000. 2. Tyers, M., Haslam, R. J., Rachubinski, R. A., Harley, C. B. Molecular analysis of pleckstrin: the major protein kinase C substrate of platelets. J. Cell. Biochem. 40: 133-145, 1989. 3. Tyers, M., Rachubinski, R. A., Stewart, M. I., Varrichio, A. M., Shorr, R. G. L., Haslam, R. J., Harley, C. B. Molecular cloning and expression of the major protein kinase C substrate of platelets. Nature 333: 470-473, 1988.

Uniprot ID: P08567

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 40 kDa

Calculated Molecular Weight: 104553 MW

Gene ID: 5341

Protein Name: Pleckstrin

Gene Full Name: pleckstrin

Synonyms: P47; Platelet 47 kDa protein; pleckstrin; PLEK