Anti-PLCD1 Antibody Picoband®

SKU: A05094-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: PLCD1

Specificity:

Background: This gene encodes a member of the phospholipase C family. Phospholipase C isozymes play critical roles in intracellular signal transduction by catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) into the second messengers diacylglycerol (DAG) and inositol triphosphate (IP3). The encoded protein functions as a tumor suppressor in several types of cancer, and mutations in this gene are a cause of hereditary leukonychia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen: E.coli-derived human PLCD1 recombinant protein (Position: Q14-R549). Human PLCD1 shares 92.2% and 92.5% amino acid (aa) sequence identity with mouse and rat PLCD1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Berridge, M. J. Inositol trisphosphate and calcium signalling. Nature 361: 315-325, 1993. 2. Cheng, H. F., Jiang, M. J., Chen, C. L., Liu, S. M., Wong, L. P., Lomasney, J. W., King, K. Cloning and identification of amino acid residues of human phospholipase C delta 1 essential for catalysis. J. Biol. Chem. 270: 5495-5505, 1995. 3. Hu, X.-T., Zhang, F.-B., Fan, Y.-C., Shu, X.-S., Wong, A. H. Y., Zhou, W., Shi, Q.-L., Tang, H.-M., Fu, L., Guan, X.-Y., Rha, S. Y., Tao, Q., He, C. Phospholipase C delta 1 is a novel 3p22.3 tumor suppressor involved in cytoskeleton organization, with its epigenetic silencing correlated with high-stage gastric cancer. Oncogene 28: 2466-2475, 2009.

Uniprot ID: P51178

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 86 kDa

Calculated Molecular Weight: 86 kDa

Gene ID: 5333

Protein Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1

Gene Full Name: phospholipase C delta 1

Synonyms: PLCD1; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1; EC 3.1.4.11; Phosphoinositide phospholipase C-delta-1; Phospholipase C-III; PLC-III; Phospholipase C-delta-1; PLC-delta-1