Anti-PLCB2 Antibody Picoband®

SKU: A05927-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: PLCB2

Specificity:

Background: 1-Phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2 is an enzyme that in humans is encoded by the PLCB2 gene. The protein encoded by this gene is a phosphodiesterase that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate to the second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol. The encoded protein is activated by G proteins and has been shown to be involved in the type 2 taste receptor signal transduction pathway. In addition, nuclear factor kappa B can regulate the transcription of this gene, whose protein product is also an important regulator of platelet responses.

Immunogen: E.coli-derived human PLCB2 recombinant protein (Position: D27-L1185). Human PLCB2 shares 85.7% and 85.3% amino acid (aa) sequence identity with mouse and rat PLCB2, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Lee, S. B., Rao, A. K., Lee, K.-H., Yang, X., Bae, Y. S., Rhee, S. G. Decreased expression of phospholipase C-b2 isozyme in human platelets with impaired function. Blood 88: 1684-1691, 1996. 2. Li, Z., Jiang, H., Xie, W., Zhang, Z., Smrcka, A. V., Wu, D. Roles of PLC-beta-2 and -beta-3 and PI3K-gamma in chemoattractant-mediated signal transduction. Science 287: 1046-1049, 2000. 3. Mao, G. F., Vaidyula, V. R., Kunapuli, S. P., Rao, A. K. Lineage-specific defect in gene expression in human platelet phospholipase C-beta-2 deficiency. Blood 99: 905-911, 2002.

Uniprot ID: Q00722

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 140 kDa

Calculated Molecular Weight: 134 kDa

Gene ID: 5330

Protein Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

Gene Full Name: phospholipase C beta 2

Synonyms: PLCB2; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2; Phosphoinositide phospholipase C-beta-2; Phospholipase C-beta-2; PLC-beta-2